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Transposable Mutagenesis for an E. coli Methionine or Histidine Synthesis Gene

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Mutating prototrophic bacteria to become auxotrophic using a transposon Making plasmid library from bacterial genome Sequencing plasmid with interrupted gene. September make plates, LB brothSeptember begin work with transposome and obtain auxotrophSeptember October 15 ligate genomic DNA, plasmid library ready October isolate kanamycin-resistant colonyOctober isolate and purify plasmidOctober November 5 enter sequence into BLASTNovember 5 to December 8 work on manuscript.

Published on Mar View 29 Download 0. Absence of bisulfite mutagenesis in the lacI gene of Escherichia coli Documents.

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A totally synthetic plasmid for general cloning, gene expression and mutagenesis in Escherichia coli Documents. Site-specific mutagenesis of Escherichia coli asparaginase II : None of the three histidine residues is required for catalysis Documents. Oligoribonucleotide synthesis. An improved synthesis of the anticodon loop region of methionine transfer ribonucleic acid from E.